Ingenio cuvettes for the ezporator electroporation system and lonzaamaxa nucleofector ii2b devices ingenio cuvettes for the ezporator electroporation system and all conventional electroporators such as biorad and harvardbtx ingenio cell droppers. We have a amaxa nucleofector lonza in the lab and it seems that this is the best way to get dna in those cells. Below, you will find the composition of the buffers and the supplement solutions. It belongs to bioresearch transfection services and produtcs category. Primary human t cells were transfected with 4 mg dna using an amaxa 4d nucleofector kit p3, hf program for unstimulated t cells. Nucleofector supplement is added to the nucleofector solution it is stable for three months at 4c. The nucleofector ii2b transfection device is the single cuvette based system that has been used in research labs since 2001, and formerly known as the amaxa device.
Amaxa, nucleofector, nucleofection and maxgfp are either. Sf cell line 96well nucleofector tm kit 960 rct for mediumthroughput transfection of cell lines, e. This nucleofector tm kit is the optimal kit for efficient transfectiion of primary natural killer nk cells. Manufacturer specifications amaxa 4d nucleofector system, lonza please note. Handmade solutions for amaxa lonza nucleofector facebook.
Cells were analyzed 4 hours post nucleofection by flow cytometry. Recommended kit sse cell line 4d nucleofector x kit amaxa 4d nucleofector protocol for hela cells for 4d nucleofector x unittransfection in suspension human cervix adenocarcinoma, adherent epithelial cell line. Due to significant diversity between different human stem cell clones, we have developed two nucleofector tm kits 1 and 2 and an easytouse nucleofector tm starter kit. The two inherited variants were also predicted as deleterious by both sift and polyphen2.
However, transfection efficiency in primary cells is low, mostly due to the high mortality rates caused by the electric pulses. Lonza walkersville p3 primary cell 4dnucleofector x kit l. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Lonza cologne ag amaxa, nucleofector, nucleofection, maxgfp, lgm3, clonetics. View detailed import data, price, monthly trends, major importing countries, major ports of lonza. Nih3t3 cells dsmz acc59 were transfected with the nucleofector kit r, program a024 and 3 g of a plasmid encoding the mouse mhc class i heavy chain molecule h2kk.
They are nonadherent or loosely adherent cells of irregular shape with typical protrusions dendrites of variable shape and length example for nucleofection of immature and mature mouse. Enhanced nucleic acid delivery using conventional electroporation devices that provide. It is also fast, so for easy to use, preconfigured kits that work especially well in primary samples, including patient sample material, it is highly recommended. In case no data are shown for the selected nucleofector platform, please take a look at our optimization strategy to get further guidance on how to easily.
The controlling unit of the 4d nucleofector tm system is suited to handle various fuctional units for efficient transfection of hardtotransfect cell lines and primary cells in different formats. Our solution is an improved electroporation technology, the nucleofector tm technology, originally introduced into the market by legacy amaxa in 2001. Lonza biologics inc offers wide range of pharma products and services which includes nucleofector devices. Specific nucleofector kits and cell typespecific solutions. The ingenio electroporation kits and solution can be used with any conventional electroporation instrument, including the amaxa nucleofector ii system. Those data are either based on lonza optimized protocols or on results shared from customers who performed an optimization based on our guidelines.
The table below shows data for the cell type and nucleofector platform selected. At lonza, we combine technological innovation with world class manufacturing and process excellence. Genomic editing, nucleofection, and the creation of nextgeneration cellbased assays. The p3 primary cell 96well nucleofectortm kit is for mediumthroughput transfection of primary cells, e. Inhouse made nucleofection buffer for efficient and cost effective transfection of raw 264. The cell line nucleofectortm kit l is one of our five nucleofectortm kits suited for transfecting cell lines in the nucleofectortm 2b device. The nucleofector tm technology uses a specific combination of optimized electrical parameters and cell typespecific solutions which enables transfer of a molecule directly into the cells nucleus.
Recommended kit sse cell line 4d nucleofector x kit amaxa 4d nucleofector protocol for mcf7 for 4d nucleofector x unittransfection in suspension human mammary gland adenocarcinoma. Nucleofector technology is based on two unique components, the nucleofector device that delivers the specifically optimized electrical parameters and nucleofector kits, which contain specific nucleofector solutions. Home lonza, technologies to enable a healthier world. Ingenio electroporation solution competitive comparison. By visiting our website or using services provided by lonza. Use ingenio electroporation solution with the lonzaamaxa nucleofector ii system for delivery of all nucleic acids. Amaxa, nucleofector, nucleofection, 4d nucleofector, nucleocuvette, maxgfp, lgm3, clonetics registeredtrademarks lonzagroup itsaliates. For mediumthroughput transfection of cell lines, e. Lonza walkersville v4xp3024 the p3 primary cell 4dnucleofector x kit l is one of five 4dnucleofector kits suited for transfecting primary cells in the 100 l nucleocuvettetm vessel format when working with the 4dnucleofectortm. For medium throughput transfection of cell lines, e. Order lonzas p3 primary cell 4dnucleofectortm x kit l on zageno. Ingenio electroporation accessories using any common electroporation instrument. Amaxa 4dnucleofector protocol for stimulated human t cells. Nucleofector technology stretching transfection dimensions.
May 04, 2004 overall, the amaxa nucleofector system and kits are extremely convenient, easy to use, and beneficial for primary cell research where nonviral gene transfer is required. This kit has been determined to be optimal for transfecting following cell types. Amaxa 4dnucleofector optimization protocol for cell lines. Used lonza amaxa nucleofector ii 3012956 electroporation. Aug 10, 2015 lonzas nucleofector technology is an improved electroporation technology that can help you achieve transfection results never before possible in primary cells, stem cells and hard to. The cell line nucleofectortm kit t is one of our five nucleofectortm kits suited for transfecting cell lines in the nucleofectortm 2b device. Nhbe nucleofector kits discontinued by 31 dec 2011. The lonza hematopoietic and immune cells knowledge center is dedicated to sharing articles and resources related to hematopoietic and immune research. Amaxa mouse dendritic cell nucleofector kit for immature and mature mouse dendritic cells progenitor cells derived from mouse bone marrow. Nmd is essential for hematopoietic stem and progenitor cells and for eliminating byproducts of programmed dna rearrangements. As mentioned above, for genome editing, engineered nucleases are used to delete, insert, or replace a gene at a targeted genomic location. Other product companynames mentioned herein respectiveowners.
Amaxa cell line nucleofector kit v for s49 mouse lymphoma. The ratio of nucleofector solution to supplement is 4. The nucleofectortm 2b device or older generations i or ii works cell type specific kits, each of them dedicated to an individual primary cell. This protocol describes an efficient method of transfecting a culture of human neural stemprecursor cells hnspcs using the nucleofector electroporation apparatus made by amaxa. Amaxa, nucleofector, nucleofection, 4d nucleofector, nucleocuvette and maxgfp. Transfection of human induced pluripotent stem cells using nucleofector technology technical reference guide technical reference guide bioresearch introduction for studying differentiation pathways in human induced pluripotent stem cells ipscs or modifying such pathways, it is important to find. Lonza, the market leader in primary cells, has expanded its human primary cell portfolio with an improved version of human umbilical vein endothelial cells huvec cultured in the absence of additive vascular endothelial growth factor vegf. Long shelf life one year shelf life allows maximum usage for your experiments. Handmade solutions for amaxa lonza nucleofector we have tried buffers to nucleofect different cell lines using the amaxa device. It includes 4dnucleofector system, 96well shuttle system, ht nucleofector system, nucleofector 2b device. Amaxa biosystems gmbh develops and commercializes gene transfer technologies and products. Together, these enable our customers to deliver their discoveries. S779n from patients and generated the constructs of mutant pogz. If you have similar experiences, please feel free to contact us so we can elaborate a common database for the transfection of.
We recently bought a 4d nucleofector, has anyone had any luck. Use ingenio with amaxa nucleofector device transfection. Ingenio electroporation solution and kits mirus bio llc. Due to this independency from cell proliferation, it allows for efficient transfection of even nondividing primary cells like resting t cells or neurons and also speeds up expression. The nucleofector device from lonza group ltd selectscience. Transfection of human induced pluripotent stem cells using nucleofector technology technical reference guide technical reference guide bioresearch introduction for studying differentiation pathways in human induced pluripotent stem cells ipscs or modifying such pathways, it is. Lonza cologne ag 50829 cologne, germany please note that the amaxa nucleofector technology is not intended to be used for diagnostic purposes or for testing or treatment in humans the nucleofector technology, comprising nucleofection process, nucleofector device, nucleofector solutions, nucleofector. Activation of human il expression involves chromatin remodeling and formation of multiple dnase ihypersensitive sites throughout the locus.
Nucleofection is an electroporationbased transfection method which enables transfer of nucleic acids such as dna and rna into cells by applying a specific voltage and reagents. Lonza walkersville p3 primary cell 4dnucleofector x kit l, 24 reactions, 100ul nucleocuvette vessels manufacturer. For further information please contact our scientific support team. The nucleofector ii device with kit components as part of the nucleofector technology, lonza provides celltype specific amaxa optimized protocols for. Product overview the cell line nucleofector tm kit l is one of our five nucleofector tm kits suited for transfecting cell lines in the nucleofector tm 2b device this kit has been determined to be optimal for transfecting following cell types. Home products molecular biology electroporation lonza amaxa nucleofector ii advanced search keywords. Genomic editing, nucleofection, and the creation of next. The nucleofector tm 2b device or older generations i or ii works cell type specific kits, each of them dedicated to an individual primary cell. Such engineered nucleases are typically comprised of two elements. Introducing a gene of interest into a cell is a powerful method for elucidating its function in vivo. It allows efficient transfection of hardtotransfect cell lines and primary cells for transfection in lowthroughput format with. Nucleofector ii general description a single cuvette based system used for the transfection of cell lines and primary cell that are hard to infect with various substrates including dna vectors or sirna olignucleotides. Inhouse made nucleofection buffer for efficient and cost.
Jurkat cells dsmz acc 282 were transfected with the cell line nucleofector kit v, program x001 and 2 g of pmaxgfp vector. Jurkat or mcf7, in the 4d nucleofectortmx unit 100 l format. As for all transfection methods, electroporation has its advantages and disadvantages. Rare inherited missense variants of pogz associate with. Nucleofection, also referred to as nucleofector technology, was invented by the biotechnology company amaxa. Nmd is essential for hematopoietic stem and progenitor. Largescale transfection using the 4d nucleofector tm lv unit experience the new functional unit for the 4d nucleofector tm system which expands our proven system to largerscale transfection.
We are a preferred global partner to the pharmaceutical, biotech and specialty ingredients markets. The nucleofector technology is a highly efficient nonviral method for transfection. For preparing aliquots, mix nucleofector solution and supplement in a ratio of 4. P3 primary cell 4dnucleofectortm x kit l description for efficient transfection of specific primary cells, e. Lonza s nucleofector technology is an improved electroporation technology that can help you achieve transfection results never before possible in primary cells, stem cells and hard to. Product overview the cell line nucleofector tm kit v is one of our five nucleofector tm kits suited for transfecting cell lines in the nucleofector tm 2b device. Amaxa basic nucleofector kit for any primary mammalian neurons primary mammalian neurons, primary neurons freshly isolated embryonic e18 or neonatal p12 mammalian neural tissues note mammalian neurons cells display significant phenotypic variations due to the wide range of both species and body sites from which they may be sourced. Nucleofector plus supplements can be used in conjunction with almost all existing nucleofector kits. Transfection of human induced pluripotent stem cells using. Transfecting human neural stem cells with the amaxa nucleofector. As part of nucleofector technology, lonza provides celltype. It enables highly efficient, transfection of primary cells, stem cells, neurons, and cell lines that have traditionally been difficult to transfect via electroporation and other nonviral. Expression of the cytokine interleukin il is critical for th2 immune responses and th2mediated allergic diseases. Dec 31, 2011 nhbe nucleofector kits discontinued by 31 dec 2011 our nucleofector kit for normal human bronchial epithelial cells nhbe will be discontinued by 31 dec 2011 and replaced by our basic nucleofector kit for primary mammalian epithelial cells vapi1005, vpi1005, vvpi1005.
Cells were analyzed 24 hours post nucleofection using light a and fluorescence microscopy b. Vca1003 size reactions 25 cell line nucleofector solution v 2. Just substitute the standard supplement delivered with regular nucleofector kit for the appropriate nucleofector plus supplement and generate your own cryopreserved readytotransfect competent cells. The cell line nucleofectortm kit v is for transfection of cell lines, e. Cell and transfection, citation, faq, culture media or use the search below.
Amaxa, nucleofector, nucleofection, maxgfp, lgm3, clonetics and biowhitakker are registered. The company offers nucleofector technology, a transfection solution which enables the transfer of various substrates, such as dna, rna, or peptides into cells. However, the culture looks like a graveyard 24h post transfection. Amaxa, nucleofector, nucleofection and maxgfp are registered. The lv unit allows for closed, scalable transfection of larger cell numbers in the range of 1x10 7 to 1x10 9 cells. On the plus side, you can use it to transfect large dna fragments and achieve good transfection efficiencies using cell lines. E1040k and two inherited missense variants with cadd scores greater than 30 p. We recently bought a 4d nucleofector, has anyone had any luck making the buffers themselves and reusing the cuvettesstrips these kits are pricey. High efficiency electroporation of hard to transfect cell lines, stem cells and. Manufacturer specifications amaxa 4dnucleofector system, lonza. We have tried buffers to nucleofect different cell lines using the amaxa device. Download a detailed overview summarising the nuclefector kits which are or will be discontinued as well as the corresponding replacement products. We recently bought a 4d nucleofector, has anyone had any. Biocompare is the leading resource for uptodate product information, product.
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